Cloning of a Cation Efflux Pump Gene Associated with Chlorhexidine Resistance in Klebsiella pneumoniae

Author:

Fang Chi-Tai1,Chen Haur-Chuan2,Chuang Yi-Ping2,Chang Shan-Chwen1,Wang Jin-Town12

Affiliation:

1. Department of Internal Medicine, National Taiwan University Hospital

2. Department of Microbiology, College of Medicine, National Taiwan University, Taipei, Taiwan

Abstract

ABSTRACT Expression libraries of a chlorhexidine-resistant Klebsiella pneumoniae strain were constructed and transformed into Escherichia coli XLOLR. Twenty chlorhexidine-resistant transformants were obtained after selection. All clones contained a novel 903-nucleotide locus. Its sequences were compatible with a cation efflux pump, and the locus was thus designated as cepA . Retransformation using cepA -containing plasmids conferred chlorhexidine resistance to both XLOLR and a chlorhexidine-sensitive K. pneumoniae strain. Therefore, CepA is associated with chlorhexidine resistance and may act as a cation efflux pump.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Reference24 articles.

1. Abbott, S. 1999. Klebsiella, Enterobacter, Citrobacter, and Serratia, p. 475-482. In P. R. Murray, E. J. Baron, M. A. Pfaller, F. C. Tenover, and R. H. Yolken (ed.), Manual of clinical microbiology, 7th ed. ASM Press, Washington, D.C.

2. The Complete Genome Sequence of Escherichia coli K-12

3. Chang, K. C., S. W. Ho, J. C. Yang, and J. T. Wang. 1997. Isolation of a genetic locus associated with metronidazole resistance in Helicobacter pylori. Biochem. Biophys. Res. Commun.236:785-788.

4. Dance, D. A., A. D. Pearson, D. V. Seal, and J. A. Lowes. 1987. A hospital outbreak caused by a chlorhexidine- and antibiotic-resistant Proteus mirabilis. J. Hosp. Infect.10:10-16.

5. Eisenetein, B. I., and D. F. Zaleznik. 2000. Enterobacteriaceae, p. 2294-2309. In G. L. Mandell, J. E. Bennett, and R. Dolin (ed.), Principles and practice of infectious diseases, 5th ed. Churchill Livingstone, New York, N. Y.

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3