Cloning of a Cation Efflux Pump Gene Associated with Chlorhexidine Resistance in Klebsiella pneumoniae

Author:

Fang Chi-Tai1,Chen Haur-Chuan2,Chuang Yi-Ping2,Chang Shan-Chwen1,Wang Jin-Town12

Affiliation:

1. Department of Internal Medicine, National Taiwan University Hospital

2. Department of Microbiology, College of Medicine, National Taiwan University, Taipei, Taiwan

Abstract

ABSTRACT Expression libraries of a chlorhexidine-resistant Klebsiella pneumoniae strain were constructed and transformed into Escherichia coli XLOLR. Twenty chlorhexidine-resistant transformants were obtained after selection. All clones contained a novel 903-nucleotide locus. Its sequences were compatible with a cation efflux pump, and the locus was thus designated as cepA . Retransformation using cepA -containing plasmids conferred chlorhexidine resistance to both XLOLR and a chlorhexidine-sensitive K. pneumoniae strain. Therefore, CepA is associated with chlorhexidine resistance and may act as a cation efflux pump.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Reference24 articles.

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