Biochemical Analysis of Metallo-β-Lactamase NDM-3 from a Multidrug-Resistant Escherichia coli Strain Isolated in Japan

Abstract

ABSTRACTNew Delhi metallo-β-lactamase-3 (NDM-3) was identified in a multidrug-resistantEscherichia coliisolate, NCGM77, obtained from the feces of a patient in Japan. The enzymatic activities of NDM-3 against β-lactams were similar to those of NDM-1, although NDM-3 showed slightly lowerkcat/Kmratios for all the β-lactams tested except for doripenem. The genetic context forblaNDM-3wastnpA-blaNDM-3-bleMBL-trpF-dsbC-tnpA-sulI-qacEdeltaI-aadA2-dfrA1, which was present on an approximately 250-kb plasmid.