Targeted Gene Disruption of the 14-α Sterol Demethylase ( cyp51A ) in Aspergillus fumigatus and Its Role in Azole Drug Susceptibility-Reference-Cited by-同舟云学术

Targeted Gene Disruption of the 14-α Sterol Demethylase ( cyp51A ) in Aspergillus fumigatus and Its Role in Azole Drug Susceptibility

Published:2005-06 Issue:6 Volume:49 Page:2536-2538
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Mellado E.¹,Garcia-Effron G.¹,Buitrago M. J.¹,Alcazar-Fuoli L.¹,Cuenca-Estrella M.¹,Rodriguez-Tudela J. L.¹

Affiliation:

1. Servicio de Micología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain

Abstract

ABSTRACT The role of Aspergillus fumigatus 14α-sterol demethylase (Cyp51A) in azole drug susceptibility was assessed. Targeted disruption of cyp51A in azole-susceptible and -resistant strains decreased MICs from 2- to 40-fold. The cyp51A mutants were morphologically indistinguishable from the wild-type strain, retaining the ability to cause pulmonary disease in neutropenic mice.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.49.6.2536-2538.2005

Reference18 articles.

1. Quantitation of Candida albicans Ergosterol Content Improves the Correlation between In Vitro Antifungal Susceptibility Test Results and In Vivo Outcome after Fluconazole Treatment in a Murine Model of Invasive Candidiasis

2. Bard, M., N. D. Lees, T. Turi, D. Craft, L. Cofrin, R. Barbuch, C. Koegel, and J. C. Loper. 1993. Sterol synthesis and viability of erg11 (cytochrome P450 lanosterol demethylase) mutations in Saccharomyces cerevisiae and Candida albicans. Lipids28:963-967.

3. Influence of Glucose Supplementation and Inoculum Size on Growth Kinetics and Antifungal Susceptibility Testing of Candida spp

4. Cullen, D., S. A. Leong, L. J. Wilson, and D. J. Henner. 1987. Transformation of Aspergillus nidulans with the hygromycin-resistance gene, hph. Gene57:21-26.

5. A Point Mutation in the 14α-Sterol Demethylase Gene cyp51A Contributes to Itraconazole Resistance in Aspergillus fumigatus

Cited by 111 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Unsaturated fatty acid perturbation combats emerging triazole antifungal resistance in the human fungal pathogen Aspergillus fumigatus;mBio;2024-07-17

2. Functional Plasticity, Redundancy, and Specificity of Lanosterol 14α-Demethylase in Regulating the Sensitivity to DMIs in Calonectria ilicicola;Journal of Agricultural and Food Chemistry;2024-04-04

3. Evolutionary history of the cytochrome P450s from Colletotrichum species and prediction of their putative functional roles during host-pathogen interactions;BMC Genomics;2024-01-12

4. Analysis of the Difenoconazole-Resistance Risk and Its Molecular Basis in Colletotrichum truncatum from Soybean;Plant Disease;2023-10-01

5. Target and non‐target site mechanisms of fungicide resistance and their implications for the management of crop pathogens;Pest Management Science;2023-09-05