Affiliation:
1. Department of Microbiology, University of Illinois at Urbana-Champaign, Urbana, Illinois
Abstract
ABSTRACT
Phenol-chloroform extraction of [
32
P]orthophosphate-labeled
E
scherichia coli
cells followed by alkaline gel electrophoresis revealed, besides the expected chromosomal DNA, two non-DNA species that we have identified as lipopolysaccharides and polyphosphates by using a combination of biochemical and genetic techniques. We used this serendipitously found straightforward protocol for direct polyphosphate detection to quantify polyphosphate levels in
E. coli
mutants with diverse defects in the DNA metabolism. We detected increased polyphosphate accumulation in the
ligA
,
ligA recBCD
,
dut ung
, and
thyA
mutants. Polyphosphate accumulation may thus be an indicator of general DNA stress.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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