Affiliation:
1. Department of Microbiology, University of Illinois at Urbana-Champaign, Urbana, Illinois
Abstract
ABSTRACT
Phenol-chloroform extraction of [
32
P]orthophosphate-labeled
E
scherichia coli
cells followed by alkaline gel electrophoresis revealed, besides the expected chromosomal DNA, two non-DNA species that we have identified as lipopolysaccharides and polyphosphates by using a combination of biochemical and genetic techniques. We used this serendipitously found straightforward protocol for direct polyphosphate detection to quantify polyphosphate levels in
E. coli
mutants with diverse defects in the DNA metabolism. We detected increased polyphosphate accumulation in the
ligA
,
ligA recBCD
,
dut ung
, and
thyA
mutants. Polyphosphate accumulation may thus be an indicator of general DNA stress.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference39 articles.
1. Ahmad, S. I., S. H. Kirk, and A. Eisenstark. 1998. Thymine metabolism and thymineless death in prokaryotes and eukaryotes. Annu. Rev. Microbiol. 52 : 591-625.
2. Akiyama, M., E. Crooke, and A. Kornberg. 1993. An exopolyphosphatase of Escherichia coli. The enzyme and its ppx gene in a polyphosphate operon. J. Biol. Chem. 268 : 633-639.
3. Amado, L., and A. Kuzminov. 2006. The replication intermediates in Escherichia coli are not the product of DNA processing or uracil excision. J. Biol. Chem. 281 : 22635-22646.
4. Construction of
Escherichia coli
K‐12 in‐frame, single‐gene knockout mutants: the Keio collection
5. Bachmann, B. J. 1987. Derivations and genotypes of some mutant derivatives of Escherichia coli K-12, p. 1190-1219. In F. C. Neidhardt (ed.), Escherichia coli and Salmonella typhimurium: cellular and molecular biology, vol. 2. American Society for Microbiology, Washington, DC.
Cited by
9 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献