Nucleotide sequence and gene-polypeptide relationships of the glpABC operon encoding the anaerobic sn-glycerol-3-phosphate dehydrogenase of Escherichia coli K-12

Author:

Cole S T1,Eiglmeier K1,Ahmed S1,Honore N1,Elmes L1,Anderson W F1,Weiner J H1

Affiliation:

1. Biochimie des Régulations Cellulaires, Institut Pasteur, Paris, France.

Abstract

The nucleotide sequence of a 4.8-kilobase SacII-PstI fragment encoding the anaerobic glycerol-3-phosphate dehydrogenase operon of Escherichia coli has been determined. The operon consists of three open reading frames, glpABC, encoding polypeptides of molecular weight 62,000, 43,000, and 44,000, respectively. The 62,000- and 43,000-dalton subunits corresponded to the catalytic GlpAB dimer. The larger GlpA subunit contained a putative flavin adenine dinucleotide-binding site, and the smaller GlpB subunit contained a possible flavin mononucleotide-binding domain. The GlpC subunit contained two cysteine clusters typical of iron-sulfur-binding domains. This subunit was tightly associated with the envelope fraction and may function as the membrane anchor for the GlpAB dimer. Analysis of the GlpC primary structure indicated that the protein lacked extended hydrophobic sequences with the potential to form alpha-helices but did contain several long segments capable of forming transmembrane amphipathic helices.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference56 articles.

1. Linkage map of Escherichia coli K-12, edition 7;Bachman B.;Microbiol. Rev.,1983

2. Multiple sequence alignment;Bacon D. J.;J. Mol. Biol.,1986

3. Buffer gradient gels and "'S-label as an aid to rapid DNA sequence determination;Biggin M. D.;Proc. Natl. Acad. Sci. USA,1983

4. Evolution and diversity in the iron-sulphur proteins;Cammack R.;Chem. Scripta,1983

5. Nucleotide sequence of the respiratory D-lactate dehydrogenase gene of Escherichia coli;Campbell H. D.;Eur. J. Biochem.,1984

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