PCR for Diagnosis of Paracoccidioidomycosis-Reference-Cited by-同舟云学术

PCR for Diagnosis of Paracoccidioidomycosis

Published:2000 Issue:9 Volume:38 Page:3478-3480
ISSN:1098-660X
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:

Author:

Gomes Glauce M.¹,Cisalpino Patrícia S.²,Taborda Carlos P.¹,de Camargo Zoilo P.¹

Affiliation:

1. Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo (UNIFESP), São Paulo, SP, 1 and

2. Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, 2 Brazil

Abstract

A PCR assay based on oligonucleotide primers derived from the sequence of the gene coding for the 43,000-Da (gp43) antigen was developed to detect Paracoccidioides brasiliensis DNA in sputa. In the standardized conditions, it could detect 10 cells/ml of sputum, providing sufficient accuracy to be useful for diagnosis of paracoccidioidomycosis.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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