Analysis of a Corynebacterium glutamicum hom gene coding for a feedback-resistant homoserine dehydrogenase

Author:

Reinscheid D J1,Eikmanns B J1,Sahm H1

Affiliation:

1. Institut für Biotechnologie, 1 des Forschungszentrums Jülich GmbH, Federal Republic of Germany.

Abstract

From a Corynebacterium glutamicum mutant possessing a homoserine dehydrogenase resistant to feedback inhibition by L-threonine, the corresponding gene (homFBR) was analyzed and compared with the wild-type hom gene. DNA fragment exchange experiments between both genes showed that a 0.23-kb region close to the 3' terminus of homFBR was responsible for deregulation. Nucleotide sequence analysis revealed a single transition from G to A in homFBR leading to replacement of glycine-378 by glutamate in the mutant homoserine dehydrogenase.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference19 articles.

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3. Amplification of three threonine biosynthesis genes in Corynebacterium glutamicum and its influence on carbon flux in different strains;Eikmanns B. J.;Appl. Microbiol. Biotechnol.,1991

4. The protein kinase family: conserved features and deduced phylogeny of the catalytic domains;Hanks S. K.;Science,1988

5. Nucleotide sequence of the gene for threonine deaminase (ILV1) of Saccharomyces cerevisiae;Kielland-Brandt M. C.;Carlsberg Res. Commun.,1984

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