Affiliation:
1. Centre de Biochimie et de Génétique Cellulaires, Centre National de la Recherche Scientifique, Toulouse, France.
Abstract
The frequency of excisive homologous recombination has been measured at various positions along the Escherichia coli chromosome. The reporter system makes use of a lambda cI857 prophage integrated by homologous recombination within Tn5 or Tn10 transposons already installed at known positions in the E. coli chromosome. The excision frequency per cell and per generation was determined by monitoring the evolution of the relative number of temperature-resistant (cured) bacteria is a function of the age of the cultures. Excisions, due to RecA-dependent homologous exchanges, appeared to occur more frequently in the preferential termination zone for chromosome replication. The highest frequency of excision observed is compatible with a recombination event at each replication cycle in this region. On the basis of these data, we propose a model involving homologous recombination in the final steps of bacterial chromosome replication and separation.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference38 articles.
1. Linkage map of Escherichia coli K-12, edition 8;Bachmann B. J.;Microbiol. Rev.,1990
2. Berg C. M. and D. E. Berg. 1987. Uses of transposable elements and maps of known insertions p. 1071-1109. In F. C. Neidhardt J. L. Ingraham K. B. Low B. Magasanik M. Schaechter and H. E. Umbarger (ed.) Escherichia coli and Salmonella typhimurium: cellular and molecular biology. American Society for Microbiology Washington D.C.
3. Physical map of a 470 x 103 base-pair region flanking the terminus of DNA replication in the Escherichia coli K12 chromosome;Bouche J. P.;J. Mol. Biol.,1982
4. Recombination deficient mutants of E. coli and other bacteria. Annu;Clark A. J.;Rev. Genet.,1973
5. A 140 base-pair DNA segment from the kanamycin resistance region of plasmid Rl acts as an origin of replication and promotes site-specific recombination;Clerget M.;J. Mol. Biol.,1984
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