Antibody-mediated immobilization of Campylobacter fetus: inhibition by a somatic antigen

Abstract

Immobilization tests were conducted on a wild-type strain of Campylobacter fetus subsp. intestinalis and on a mutant lacking an antiphagocytic cell surface component. Highly effective immobilization of the mutant, both as single cells and clumps of cells, was produced with an antiserum containing antibodies specific for the flagellar hook and filament and for the O antigen. Damage to flagellar hooks after reaction with this antiserum was observed only with cells of the mutant. Single-cell immobilization of the mutant was also produced with an antiserum specific for a heat-stable somatic antigen which was distinct from the O antigen and was exposed on the cell surface only of the mutant. Minimal immobilization of the wild strain was brought about by either of these antisera. It was shown also that O antibodies had no effect on the motility of either the wild strain or mutant. These findings indicate that antibody-mediated immobilization may be brought about by effects on the flagellar hook or cell body, as well as on the flagellar filament. Furthermore, the protection from immobilization afforded the bacterium by the antiphagocytic surface structure suggests a dual function for this virulence factor in the infected animal.