Purification of Intact Flagella from Escherichia coli and Bacillus subtilis-Reference-Cited by-同舟云学术

Purification of Intact Flagella from Escherichia coli and Bacillus subtilis

Published:1971-01 Issue:1 Volume:105 Page:376-383
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

DePamphilis M. L.¹,Adler Julius²

Affiliation:

1. Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706

2. Department of Genetics, University of Wisconsin, Madison, Wisconsin 53706

Abstract

A procedure is described for the purification of bacterial flagella in the form of a filament-hook-basal body complex (intact flagella) free from detectable cell wall, membrane, or cytoplasmic material. Spheroplasts produced with lysozyme and ethylenediaminetetraacetic acid were lysed with Triton X-100, and the flagella were purified by (NH 4 ) 2 SO 4 precipitation, differential centrifugation, and CsCl gradient centrifugation. As much as 40% of the flagella were recovered, and they contained about one basal body per 4 to 6 μm of flagella. The same procedure developed for Escherichia coli was also successful for purifying intact flagella from Bacillus subtilis .

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jb.105.1.376-383.1971

Reference25 articles.

1. In vitro formation of flagella-like filaments and other structures from flagellin;Abram D.;J. Mol. Biol.,1964

2. Basal structure and attachment of flagella in cells of Proteus vulgaris;Abram D.;J. Bacteriol.,1965

3. Differentiation within the bacterial flagellum and isolation of the proximal hook;Abram D.;J. Bacteriol.,1970

4. Genetics of motility in E. coli: complementation of paralyzed mutants;Armstrong J. B.;Genetics,1967

5. Campbell D. H. 1964. Methods in immunology; a laboratory text for instruction and research p. 122-124. Benjamin Co. Inc. New York.

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