Effects of nutrient deprivation on Vibrio cholerae

Author:

Baker R M,Singleton F L,Hood M A

Abstract

Environmental and clinical strains of Vibrio cholerae were exposed to nutrient-free artificial seawater and filtered natural seawater microcosms for selected time intervals and examined for changes in cell morphology and number. Cells observed by transmission electron and epifluorescence microscopy were found to undergo gross alterations in cell morphology with time of exposure. The vibroid cells decreased in volume by 85% and developed into small coccoid forms surrounded by remnant cell walls. The initial number of cells inoculated into nutrient-free microcosms (culturable count and direct viable count) increased 2.5 log10 within 3 days, and even after 75 days the number of viable cells was still 1 to 2 log10 higher than the initial inoculum size. Nutrient-depleted coccoid-shaped cells were restored to normal size and assumed a bacillary shape within 3 h and began to divide within 5 h after nutrient supplementation. The increase in cell number and decrease in cell volume under nutrient-depleted conditions, as well as the rapid growth response after nutrient supplementation, may describe some of the survival mechanisms of V. cholerae in the aquatic environment.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference28 articles.

1. Recovery from nutrient starvation by a marine Vibrio sp. Appi;Amy P. S.;Environ. Microbiol.,1983

2. Changes in morphology and cell wall structure that occur during growth of Vibrio sp. NCTC4716 in batch culture;Baker D. A.;J. Gen. Microbiol.,1975

3. Baker R. M. M. T. MacDonell F. L. Singleton and M. A. Hood. 1982. Effects of surface association and osmolarity of Vibrio cholerae p. 224-238. In R. Nickelson (ed.) Proceedings of the Seventh Annual Tropical and Subtropical Fisheries Conference of the Americas. Texas A&M University College Station.

4. Initiation and termination of DNA replication after amino acid starvation of E. coli 15 T-;Bird R.;Cold Spring Harbor Symp. Quant. Biol.,1968

5. Factors affecting the survival of bacteria in sea water;Carlucci A. F.;Appl. Microbiol.,1959

Cited by 141 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3