Multicenter Validation of the cppB Gene as a PCR Target for Detection of Neisseria gonorrhoeae-Reference-Cited by-同舟云学术

Multicenter Validation of the cppB Gene as a PCR Target for Detection of Neisseria gonorrhoeae

Published:2004-09 Issue:9 Volume:42 Page:4332-4334
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Bruisten S. M.¹,Noordhoek G. T.²,van den Brule A. J. C.³,Duim B.⁴,Boel C. H. E.³,El-Faouzi K.¹,du Maine R.⁴,Mulder S.²,Luijt D.⁵,Schirm J.⁵

Affiliation:

1. Municipal Health Service, GG&GD

2. Public Health Laboratory Friesland, LVF, Leeuwarden

3. Laboratory for Pathology and Microbiology, PAMM, Veldhoven

4. Department of Medical Microbiology, AMC, Amsterdam

5. Regional Public Health Laboratory, SLGD, Groningen, The Netherlands

Abstract

ABSTRACT The cppB gene is often used as a target for detection of Neisseria gonorrhoeae by PCR. Using a coded panel of 500 DNA samples, we determined that the cppB gene is missing in 5.8% of N. gonorrhoeae strains, and therefore we consider the cppB gene to be an unsuitable target.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.42.9.4332-4334.2004

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