Affiliation:
1. Laboratory of Bacterial Cell Biology, Instituto de Tecnologia Química e Biológica (ITQB), Universidade Nova de Lisboa (UNL), Oeiras, Portugal
Abstract
ABSTRACT
Genetic manipulation of
Staphylococcus aureus
is limited by the availability of only a single strain, RN4220, that is capable of easily accepting foreign DNA. Inactivation of the
hsdR
gene of the SauI type I restriction-modification system was shown previously to be responsible for the high transformation efficiency of RN4220 (D. E. Waldron and J. A. Lindsay, J Bacteriol.
188:
5578-5585, 2006). However, deletion of this gene in three different
S. aureus
strains was not sufficient to make them readily transformable, which would be remarkably useful for genetic studies of this pathogenic organism. These results indicate that another unknown factor(s) is required for the transformable phenotype in
S. aureus
.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
44 articles.
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