Affiliation:
1. The Center for Environmental Biotechnology1 and
2. Department of Civil and Environmental Engineering,2 The University of Tennessee, Knoxville, Tennessee 37996-1605
Abstract
ABSTRACT
A bioluminescent reporter strain, Ralstonia eutrophaENV307(pUTK60), was constructed for the detection of polychlorinated biphenyls by inserting the biphenyl promoter upstream of the bioluminescence genes. In the presence of a nonionic surfactant, which enhances the solubility of chlorinated biphenyls, bioluminescence was induced three- to fourfold over background by biphenyl, monochlorinated biphenyls, and Aroclor 1242. The minimum detection limits for these compounds ranged from 0.15 mg/liter for 4-chlorobiphenyl to 1.5 mg/liter for Aroclor 1242.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
73 articles.
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