Spoligotyping of Mycobacterium tuberculosis Complex Isolates by Use of Ligation-Based Amplification and Melting Curve Analysis

Author:

Zeng Xiaohong123,Li Hui4,Zheng Rongrong5,Kurepina Natalia6,Kreiswirth Barry N.6,Zhao Xilin26,Xu Ye123,Li Qingge123

Affiliation:

1. Engineering Research Centre of Molecular Diagnostics, Ministry of Education, State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiamen, Fujian, China

2. State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen, Fujian, China

3. Engineering Research Centre of Personalized Molecular Diagnostics of Xiamen, Fujian, China

4. Tuberculosis Reference Laboratory, Henan Center for Disease Control and Prevention, Zhengzhou, Henan, China

5. Division of Tuberculosis Control and Prevention, Xiamen Center for Disease Control and Prevention, Xiamen, Fujian, China

6. Public Health Research Institute, New Jersey Medical School, Rutgers University, Newark, New Jersey, USA

Abstract

ABSTRACT We report here a ligation-based spoligotyping that can identify unamplified spacers in membrane-based spoligotyping due to asymmetric insertion of IS 6110 in the direct repeat locus. Our typing yielded 84.4% (411/487) concordance with traditional typing and 100% (487/487) accuracy when confirmed by DNA sequencing.

Funder

National High Technology Research and Development Program of China

National Natural Science Foundation of China

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3