Affiliation:
1. NIZO food research, P.O. Box 20, 6710 BA Ede, The Netherlands
2. Plant Research International, P.O. Box 16, 6700 AA Wageningen, The Netherlands
Abstract
ABSTRACT
Lactic acid bacteria, such as
Lactococcus lactis
, are attractive hosts for the production of plant-bioactive compounds because of their food grade status, efficient expression, and metabolic engineering tools. Two genes from strawberry (
Fragaria x ananassa
), encoding an alcohol acyltransferase (SAAT) and a linalool/nerolidol synthase (FaNES), were cloned in
L. lactis
and actively expressed using the nisin-induced expression system. The specific activity of SAAT could be improved threefold (up to 564 pmol octyl acetate h
−1
mg protein
−1
) by increasing the concentration of tRNA
1
Arg
, which is a rare tRNA molecule in
L. lactis
. Fermentation tests with GM17 medium and milk with recombinant
L. lactis
strains expressing SAAT or FaNES resulted in the production of octyl acetate (1.9 μM) and linalool (85 nM) to levels above their odor thresholds in water. The results illustrate the potential of the application of
L. lactis
as a food grade expression platform for the recombinant production of proteins and bioactive compounds from plants.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
38 articles.
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