Affiliation:
1. Milstead Laboratory of Chemical Enzymology, “Shell” Research, Ltd., Sittingbourne, Kent, England
Abstract
An apparatus for the continuous and automatic measurement of respiration during growth of micro-organisms in stirred aerated culture is described. The effluent atmosphere from the culture vessels is passed through commercially available oxygen and carbon dioxide gas analyzers, and their electrical output is fed to a multipoint recorder. The apparatus has been used to measure the respiration of
Escherichia coli, Pseudomonas aeruginosa, Bacillus thuringiensis,
and
Methylococcus capsulatus
during growth on complex and defined media. In addition,
p
H values and dissolved oxygen concentrations were measured. Growth of
P. thuringiensis
in glucose-limited media showed unexpected interruptions in the oxygen consumption curves which resembled diauxic growth; growth of
B. thuringiensis
in complex media showed similar discontinuities of respiration. These results are explained as the sequential utilization of preferred substrates which, in the case of
P. aeruginosa
were provided as transient intermediates of glucose.
Publisher
American Society for Microbiology
Subject
General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine
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