Simultaneous Determination of Mycobacterium leprae Drug Resistance and Single-Nucleotide Polymorphism Genotype by Use of Nested Multiplex PCR with Amplicon Sequencing-Reference-Cited by-同舟云学术

Simultaneous Determination of Mycobacterium leprae Drug Resistance and Single-Nucleotide Polymorphism Genotype by Use of Nested Multiplex PCR with Amplicon Sequencing

Published:2021-09-20 Issue:10 Volume:59 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Iwao Yasuhisa¹²,Mori Shuichi¹,Ato Manabu¹^ORCID,Nakata Noboru¹²^ORCID

Affiliation:

1. Department of Mycobacteriology, Leprosy Research Center, National Institute of Infectious Diseases, Higashimurayama, Tokyo, Japan

2. Antimicrobial Resistance Research Center, National Institute of Infectious Diseases, Higashimurayama, Tokyo, Japan

Abstract

Mycobacterium leprae is the predominant cause of leprosy worldwide, and its genotypes can be classified into four single-nucleotide polymorphism (SNP) types and 16 subtypes. Determining M. leprae drug resistance and genotype is typically done by PCR and Sanger DNA sequencing, which require substantial effort.

Funder

MEXT | Japan Society for the Promotion of Science

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00814-21

Reference39 articles.

1. Leprosy

2. Global leprosy (Hansen disease) update, 2019: time to step-up prevention initiatives;WHO;Wkly Epidemiol Rec,2020

3. A NewMycobacteriumSpecies Causing Diffuse Lepromatous Leprosy