Carryover Contamination-Controlled Amplicon Sequencing Workflow for Accurate Qualitative and Quantitative Detection of Pathogens: a Case Study on SARS-CoV-2-Reference-Cited by-同舟云学术

Carryover Contamination-Controlled Amplicon Sequencing Workflow for Accurate Qualitative and Quantitative Detection of Pathogens: a Case Study on SARS-CoV-2

Published:2023-06-15 Issue:3 Volume:11 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Gao Lifen¹,Li Lun¹,Fang Bin²,Fang Zhiwei¹,Xiang Yanghai³,Zhang Min³,Zhou Junfei¹,Song Huiyin¹,Chen Lihong¹,Li Tiantian¹,Xiao Huafeng¹,Wan Renjing¹,Jiang Yongzhong²,Peng Hai¹⁴^ORCID

Affiliation:

1. Institute for Systems Biology, Jianghan University, Wuhan, Hubei, People’s Republic of China

2. Hubei Provincial Centers for Disease Control and Prevention, Wuhan, Hubei, People’s Republic of China

3. Yueyang Central Hospital, Yueyang, Hunan, People’s Republic of China

4. Mingliao Biotechnology Co., Ltd., Wuhan, Hubei, People’s Republic of China

Abstract

Accuracy, a key indicator of pathogen detection technology, is compromised by carryover contamination in the amplicon sequencing workflow. Taking the detection of SARS-CoV-2 as case, this study presents a new carryover contamination-controlled amplicon sequencing workflow.

Funder

Wuhan Science and Technology Project

Standardization Administration of China

Key R&D Projects of Science and Technology Department of Hubei Province

Natural Science Foundation of Hubei Province

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.00206-23

Reference29 articles.