Affiliation:
1. Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, Maryland 20742
Abstract
ABSTRACT
The photosynthetic cyanobacterium
Synechocystis
sp. strain PCC6803 possesses homologs of known genes of the non-mevalonate 2-
C
-methyl-
d
-erythritol 2-phosphate (MEP) pathway for synthesis of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Isoprenoid biosynthesis in extracts of this cyanobacterium, measured by incorporation of radiolabeled IPP, was not stimulated by pyruvate, an initial substrate of the MEP pathway in
Escherichia coli
, or by deoxyxylulose-5-phosphate, the first pathway intermediate in
E. coli
. However, high rates of IPP incorporation were obtained with addition of dihydroxyacetone phosphate (DHAP) and glyceraldehyde 3-phosphate (GA3P), as well as a variety of pentose phosphate cycle compounds. Fosmidomycin (at 1 μM and 1 mM), an inhibitor of deoxyxylulose-5-phosphate reductoisomerase, did not significantly inhibit phototrophic growth of the cyanobacterium, nor did it affect [
14
C]IPP incorporation stimulated by DHAP plus GA3P. To date, it has not been possible to unequivocally demonstrate IPP isomerase activity in this cyanobacterium. The combined results suggest that the MEP pathway, as described for
E. coli
, is not the primary path by which isoprenoids are synthesized under photosynthetic conditions in
Synechocystis
sp. strain PCC6803. Our data support alternative routes of entry of pentose phosphate cycle substrates derived from photosynthesis.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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