Affiliation:
1. Institute of Biochemistry, Academic Hospital Centre, Justus Liebig University, D-35392 Giessen, Germany
Abstract
ABSTRACT
In a variety of organisms, including plants and several eubacteria, isoprenoids are synthesized by the mevalonate-independent 2-
C
-methyl-
d
-erythritol 4-phosphate (MEP) pathway. Although different enzymes of this pathway have been described, the terminal biosynthetic steps of the MEP pathway have not been fully elucidated. In this work, we demonstrate that the
gcpE
gene of
Escherichia coli
is involved in this pathway.
E. coli
cells were genetically engineered to utilize exogenously provided mevalonate for isoprenoid biosynthesis by the mevalonate pathway. These cells were then deleted for the essential
gcpE
gene and were viable only if the medium was supplemented with mevalonate or the cells were complemented with an episomal copy of
gcpE
.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference41 articles.
1. Tools for discovery of inhibitors of the 1-deoxy-d-xylulose 5-phosphate (DXP) synthase and DXP reductoisomerase: an approach with enzymes from the pathogenic bacterium Pseudomonas aeruginosa;Altincicek B.;FEMS Microbiol. Lett.,2000
2. Dimethylallyl pyrophosphate is not the committed precursor of isopentenyl pyrophosphate during terpenoid biosynthesis from 1-deoxyxylulose in higher plants;Arigoni D.;Proc. Natl. Acad. Sci. USA,1999
3. Ausubel
F. M.
Brent
R.
Kingston
R. E.
Moore
D. D.
Seidman
J. G.
Smith
J. A.
Struhl
K.
Current protocols in molecular biology.
1987
John Wiley & Sons Inc.
New York N.Y
4. Sequence and characterization of the gcpE gene of Escherichia coli;Baker J.;FEMS Microbiol. Lett.,1992
5. Allelic exchange in Escherichia coli using the Bacillus subtilis sacB gene and a temperature-sensitive pSC101 replicon;Blomfield I. C.;Mol. Microbiol.,1991
Cited by
70 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献