Affiliation:
1. Department of Biology, University of Michigan, Ann Arbor, Michigan 48109-1048
Abstract
ABSTRACT
About 10% of the nalidixic acid-resistant (Nal
r
) mutants in a transposition-induced library exhibited a growth factor requirement as the result of
cysH
,
icdA
,
metE
, or
purB
mutation. Resistance in all of these mutants required a functional AcrAB-TolC efflux pump, but the EmrAB-TolC pump played no obvious role. Transcription of
acrAB
was increased in each type of Nal
r
mutant. In the
icdA
and
purB
mutants, each of the known signaling pathways appeared to be used in activating the AcrAB-TolC pump. The metabolites that accumulate upstream of the blocks caused by the mutations are hypothesized to increase the levels of the AcrAB-TolC pump, thereby removing nalidixic acid from the organism.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
73 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献