Affiliation:
1. Lehrstuhl für Mikrobiologie, Institut für Mikrobiologie, Biochemie und Genetik der Friedrich-Alexander-Universität Erlangen-Nürnberg, D-91058 Erlangen, Germany
Abstract
ABSTRACT
Bacillus subtilis ccpA
mutant strains exhibit two distinct phenotypes: they are defective in catabolite repression, and their growth on minimal media is strongly impaired. This growth defect is largely due to a lack of expression of the
gltAB
operon. However, growth is impaired even in the presence of glutamate. Here, we demonstrate that the
ccpA
mutant strain needs methionine and the branched-chain amino acids for optimal growth. The control of expression of the
ilv-leu
operon by CcpA provides a novel regulatory link between carbon and amino acid metabolism.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference24 articles.
1. Positive regulation of glutamate biosynthesis in Bacillus subtilis
2. Deutscher J. A. Galinier and I. Martin-Verstraete. 2002. Carbohydrate uptake and metabolism p. 129-150. In A. L. Sonenshein J. A. Hoch and R. Losick (ed.) Bacillus subtilis and its closest relatives: from genes to cells. American Society for Microbiology Washington D.C.
3. Faires, N., S. Tobisch, S. Bachem, I. Martin-Verstraete, M. Hecker, and J. Stülke. 1999. The catabolite control protein CcpA controls ammonium assimilation in Bacillus subtilis. J. Mol. Microbiol. Biotechnol.1:141-148.
4. Fillinger, S., S. Boschi-Muller, S. Azza, E. Dervyn, G. Branlant, and S. Aymerich. 2000. Two glyceraldehyde-3-phosphate dehydrogenases with opposite physiological roles in a nonphotosynthetic bacterium. J. Biol. Chem.275:14031-14037.
5. Fisher S. H. and M. Débarbouillé. 2002. Nitrogen source utilization and its regulation p. 181-191. In A. L. Sonenshein J. A. Hoch and R. Losick (ed.) Bacillus subtilis and its closest relatives: from genes to cells. American Society for Microbiology Washington D.C.