Affiliation:
1. Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111
Abstract
ABSTRACT
CodY is a global transcriptional regulator that is known to control directly the expression of at least two dozen operons in
Bacillus subtilis
, but the rules that govern the binding of CodY to its target DNA have been unclear. Using DNase I footprinting experiments, we identified CodY-binding sites upstream of the
B. subtilis ylmA
and
yurP
genes. The protected regions overlapped versions of a previously proposed CodY-binding consensus motif, AATTTTCWGAAAATT. Multiple single mutations were introduced into the CodY-binding sites of the
ylmA, yurP, dppA
, and
ilvB
genes. The mutations affected both the affinity of CodY for its binding sites in vitro and the expression in vivo of
lacZ
fusions that carry these mutations in their promoter regions. Our results show that versions of the AATTTTCWGAAAATT motif, first identified for
Lactococcus lactis
CodY, with up to five mismatches play an important role in the interaction of
B. subtilis
CodY with DNA.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
83 articles.
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