Affiliation:
1. Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111, USA.
Abstract
The azlB locus of Bacillus subtilis was defined previously by a mutation conferring resistance to a leucine analog, 4-azaleucine (J. B. Ward, Jr., and S. A. Zahler, J. Bacteriol. 116:727-735, 1973). In this report, azlB is shown to be the first gene of an operon apparently involved in branched-chain amino acid transport. The product of the azlB gene is an Lrp-like protein that negatively regulates expression of the azlBCDEF operon. Resistance to 4-azaleucine in azlB mutants is due to overproduction of AzlC and AzlD, two novel hydrophobic proteins.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference47 articles.
1. pBluescript II: multifunctional cloning and mapping vectors;Alting-Mees M. A.;Methods Enzymol.,1992
2. Basic local alignment search tool;Altschul S. F.;J. Mol. Biol.,1990
3. Anagnostopoulos C. P. J. Piggot and J. A. Hoch. 1993. The genetic map of Bacillus subtilis p. 425-461. In A. L. Sonenshein J. A. Hoch and R. Losick (ed.) Bacillus subtilis and other gram-positive bacteria: biochemistry physiology and molecular genetics. American Society for Microbiology Washington D.C.
4. Belitsky B. R. Unpublished data.
5. Altered transcription activation specificity of a mutant form of Bacillus subtilis GltR, a LysR family member;Belitsky B. R.;J. Bacteriol.,1997
Cited by
77 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献