Evaluation of Five Commercial Real-Time PCR Assays for Detection of Mycoplasma pneumoniae in Respiratory Tract Specimens-Reference-Cited by-同舟云学术

Evaluation of Five Commercial Real-Time PCR Assays for Detection of Mycoplasma pneumoniae in Respiratory Tract Specimens

Published:2009-07 Issue:7 Volume:47 Page:2269-2271
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Touati A.¹,Benard A.²,Hassen A. Ben¹,Bébéar C. M.³,Pereyre S.³

Affiliation:

1. Service des Laboratoires, Centre National de Greffe de Moelle Osseuse, Tunis, Tunisia

2. Unité de Soutien Méthodologique à la Recherche Clinique et Epidémiologique, INSERM U897, CIC-EC 7, Université Victor Segalen Bordeaux 2 and CHU de Bordeaux, Bordeaux, France

3. Laboratoire de Bactériologie EA 3671, Université Victor Segalen Bordeaux 2 and CHU de Bordeaux, Bordeaux, France

Abstract

ABSTRACT The performances of five commercial TaqMan real-time PCR assays for the detection of Mycoplasma pneumoniae in respiratory tract specimens were evaluated in comparison with an in-house real-time PCR. All kits allowed prompt and specific results, validated by the use of an internal control. The Nanogen kit showed the best clinical sensitivity.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00326-09

Reference18 articles.

1. Comparison of a New Quantitative ompA -Based Real-Time PCR TaqMan Assay for Detection of Chlamydia pneumoniae DNA in Respiratory Specimens with Four Conventional PCR Assays

2. Atkinson, T. P., M. F. Balish, and K. B. Waites. 2008. Epidemiology, clinical manifestations, pathogenesis and laboratory detection of Mycoplasma pneumoniae infections. FEMS Microbiol. Rev.32:956-973.

3. Daxboeck, F., G. Khanakah, C. Bauer, M. Stadler, H. Hoffmann, and G. Stanek. 2005. Detection of Mycoplasma pneumoniae in serum specimens from patients with Mycoplasma pneumoniae by PCR. Int. J. Med. Microbiol.295:279-285.

4. Daxboeck, F., R. Krause, and C. Wenisch. 2003. Laboratory diagnosis of Mycoplasma pneumoniae infection. Clin. Microbiol. Infect.9:263-273.

5. de Barbeyrac, B., C. Bernet-Poggi, F. Fèbrer, H. Renaudin, M. Dupon, and C. Bébéar. 1993. Detection of Mycoplasma pneumoniae and Mycoplasma genitalium in clinical samples by polymerase chain reaction. Clin. Infect. Dis.17(Suppl. 1):S83-S89.

Cited by 37 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Mycoplasma pneumoniae detections before and during the COVID-19 pandemic: results of a global survey, 2017 to 2021;Eurosurveillance;2022-05-12

2. Mycoplasma and Ureaplasma;Molecular Typing in Bacterial Infections, Volume I;2022

3. Multi-frequency impedance sensing for detection and sizing of DNA fragments;Scientific Reports;2021-03-22

4. Evaluation of Commercial Molecular Diagnostic Methods for Detection and Determination of Macrolide Resistance in Mycoplasma pneumoniae;Journal of Clinical Microbiology;2020-05-26

5. Evaluation of the ELITe InGenius PCR Platform for Detection of Mycoplasma pneumoniae;Journal of Clinical Microbiology;2019-06