Evaluation of Commercial Molecular Diagnostic Methods for Detection and Determination of Macrolide Resistance in Mycoplasma pneumoniae

Author:

Leal Sixto M.1,Totten Arthur H.2,Xiao Li3,Crabb Donna M.2,Ratliff Amy2,Duffy Lynn B.1,Fowler Karen B.2,Mixon Emily2,Winchell Jonas M.4,Diaz Maureen H.4,Benitez Alvaro J.4,Wolff Bernard J.4ORCID,Qin Xuan5,Tang Y.-W.6ORCID,Gonzalez Mark7,Selvarangan Raj8,Hong Tao9,Brooks Edward10,Dallas Steve11,Atkinson T. Prescott2,Zheng Xiaotian12ORCID,Dien Bard Jennifer13,Waites Ken B.1

Affiliation:

1. Department of Pathology, University of Alabama at Birmingham, Birmingham, Alabama, USA

2. Department of Pediatrics, University of Alabama at Birmingham, Birmingham, Alabama, USA

3. Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama, USA

4. Division of Bacterial Diseases, Respiratory Diseases Branch, National Center for Immunization and Respiratory Diseases, Centers for Disease Control & Prevention, Atlanta, Georgia, USA

5. Department of Laboratory Medicine and Pathology, Seattle Children’s Hospital, Seattle, Washington, USA

6. Memorial Sloan Kettering Cancer Center, Weill Medical College of Cornell University, New York, New York, USA

7. Children’s Healthcare of Atlanta, Emory University, Atlanta, Georgia, USA

8. Children’s Mercy Hospital, Kansas City, Missouri, USA

9. Department of Pathology, Hackensack University Medical Center, Hackensack, New Jersey, USA

10. Department of Pediatrics, University of Texas Health Science Center, San Antonio, Texas, USA

11. Department of Pathology, University of Texas Health Science Center, San Antonio, Texas, USA

12. Ann and Robert H. Lurie Children’s Hospital of Chicago, Chicago, Illinois, USA

13. Department of Pathology and Laboratory Medicine, Children’s Hospital of Los Angeles, Keck School of Medicine, University of Southern California, Los Angeles, California, USA

Abstract

We evaluated six commercial molecular tests targeting Mycoplasma pneumoniae , namely, the BioFire FilmArray respiratory panel (RP), the Meridian Alethia Mycoplasma Direct, the GenMark ePlex respiratory pathogen panel (RPP), the Luminex NxTAG RPP, the ELITech ELITe InGenius Mycoplasma MGB research use only (RUO) PCR, and the SpeeDx Resistance Plus MP assays. Laboratory-developed PCR assays at the University of Alabama at Birmingham and the Centers for Disease Control and Prevention were used as reference standards.

Funder

HHS | Centers for Disease Control and Prevention

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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