Anhydrobiotic Engineering of Gram-Negative Bacteria

Author:

García de Castro Arcadio1,Bredholt Harald2,Strøm Arne R.2,Tunnacliffe Alan1

Affiliation:

1. Institute of Biotechnology, University of Cambridge, Cambridge CB2 1QT, United Kingdom,1and

2. Department of Biotechnology, Norwegian University of Science and Technology, N-7491 Trondheim, Norway2

Abstract

ABSTRACT Anhydrobiotic engineering aims to improve desiccation tolerance in living organisms by adopting the strategies of anhydrobiosis. This was achieved for Escherichia coli and Pseudomonas putida by osmotic induction of intracellular trehalose synthesis and by drying from trehalose solutions, resulting in long-term viability in the dried state.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference13 articles.

1. Engineering Desiccation Tolerance in Escherichia coli

2. Anhydrobiosis;Crowe J. H.;Annu. Rev. Physiol.,1992

3. The role of vitrification in anhydrobiosis;Crowe J. H.;Annu. Rev. Physiol.,1998

4. Mannitol-enhanced survival of Lactococcus lactis subjected to drying;Efiuvwevwere B. J. O.;Appl. Microbiol. Biotechnol.,1999

5. Role of the trehalose carrier in dehydration resistance of Saccharomyces cerevisiae;Eleutherio E. C. A.;Biochim. Biophys. Acta,1993

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