Associative Growth Studies in Three-Strain Mixtures of Lactic Streptococci

Author:

Reddy M. S.1,Vedamuthu E. R.1,Washam C. J.1,Reinbold G. W.1

Affiliation:

1. Department of Food Technology, Iowa State University, Ames, Iowa 50010

Abstract

A recently developed differential agar medium was used to study associative growth patterns in 17 different heterologous, three-strain mixtures of Streptococcus lactis, S. cremoris , and S. diacetilactis grown in milk. Mixtures were made by combining equal volumes of 18-hr milk cultures of the three species. Relative populations of component species were followed through three successive transfers in milk after the initial mixed propagation. Direct evidence for strain dominance and compatibility was obtained. A procedure also was developed to estimate the extent of suppression of S. lactis and S. diacetilactis in a mixture containing a dominant S. cremoris strain. The technique described could be successfully applied in quality-control work in the dairy-starter manufacturing industry.

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Reference14 articles.

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2. Domination among strains of lactic streptococci with attention to antibiotic production;Collins E. B.;Appl. Microbiol.,1961

3. Compatibility amongst strains of lactic streptococci;Czulak J.;Aust. J. Dairy Technol.,1954

4. Bacteriology of butter cultures: a review;Hammer B. W.;J. Dairy Sci.,1943

5. Citrate utilization and overgrowth by Streptococcus diacetilactis in mixed strain starter cultures;Henning D. R.;J. Dairy Sci.,1964

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