Author:
Chen Liang,Chavda Kalyan D.,Mediavilla José R.,Zhao Yanan,Fraimow Henry S.,Jenkins Stephen G.,Levi Michael H.,Hong Tao,Rojtman Albert D.,Ginocchio Christine C.,Bonomo Robert A.,Kreiswirth Barry N.
Abstract
ABSTRACTWe describe a multiplex real-time PCR assay capable of identifying both the epidemicKlebsiella pneumoniaeST258 clone andblaKPCcarbapenemase genes in a single reaction. The assay displayed excellent sensitivity (100%) and specificity (100%) for identification of ST258 clone andblaKPCin a collection of 75K. pneumoniaeisolates comprising 41 sequence types. Our results suggest that this assay is an effective tool for surveillance of this clone among carbapenem-resistantK. pneumoniaeclinical isolates.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Pharmacology (medical),Pharmacology
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