Affiliation:
1. Food Research Laboratories, Health Protection Branch, Health and Welfare Canada, Ottawa, Canada, K1A 0L2
Abstract
The Shahidi-Ferguson perfringens, tryptose-sulfite-cycloserine (TSC), and egg yolk-free TSC agars have been tested for their suitability to enumerate fecal spores of
Clostridium perfringens
. When these spores comprised at least 20% of the total anaerobe spores, equally accurate counts were obtained in the three media. With lower ratios of
C. perfringens
spores, the most accurate counts were obtained in egg yolk-free TSC agar. The median
C. perfringens
spore count of 60 normal fecal specimens was log 3.4/g. A nonmotile, sulfite- and nitrate-reducing
Clostridium
, not identifiable with any known clostridial species, was isolated from 14 out of 60 fecal specimans. It was not differentiated from
C. perfringens
in the nitrite motility test, but could be distinguished by its inability to liquefy gelatin.
Publisher
American Society for Microbiology
Subject
General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine
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