Affiliation:
1. Connaught Medical Research Laboratories, University of Toronto, Toronto, Ontario, Canada
Abstract
14
C-labeled tuberculin purified protein derivative (
14
C-tuberculin PPD) has been prepared from culture filtrates of
Mycobacterium tuberculosis
var.
hominis
grown in a culture medium containing uniformly labeled
14
C-amino acids. With a mixture of
14
C-amino acids (an acid hydrolysate of
14
C-
Chlorella
protein) in the medium, the recoveries of
14
C in the final product were higher than with
14
C-labeled-
l
-glutamic acid.
14
C-tuberculin PPD was separated into tuberculoprotein and nucleic acid by paper electrophoresis. The specific radioactivity of tuberculoprotein was substantially greater than that of the nucleic acid.
14
C-tuberculin PPD is advocated as a means to measure the adsorption of tuberculin to glass or other surfaces. It could also prove useful as a means to study the structure and mode of action of tuberculin.
Publisher
American Society for Microbiology
Subject
General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine
Reference4 articles.
1. BLOCK R. J. E. L. DURRUM AND G. ZWEIG. 1958. Paper chromatography and paper electrophoresis p. 68-71. Academic Press Inc. New York.
2. Preparation, purification, and stability of tuberculin;LANDI S.;Appl. Microbiol.,1963
3. Physicochemical and biological studies on various preparations of tuberculin purified protein derivative;LANDI S.;Appl. Microbiol.,1965
4. Adsorption of tuberculin PPD to glass and plastic surfaces;LANDI S., H.;Bull. World Health Organ.,1966
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1 articles.
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