Constitutive expression of the iron-enterochelin and ferrichrome uptake systems in a mutant strain of Salmonella typhimurium

Abstract

Two high-affinity iron uptake systems are known in Salmonella typhimurium, one utilizing iron-enterochelin and the other utilizing ferrichrome. It has been shown previously that expression of several elements of the iron-enterochelin uptake system are regulated by the iron content of the medium, with growth in high-iron medium resulting in repression of enzymes of enterochelin synthesis and degradation and of the ability of whole cells to take up iron-enterochelin. In this study we describe a mutant strain in which growth in high-iron medium was associated with constitutive expression of: (i) iron-enterochelin uptake by whole cells; (ii) ferrichrome uptake by whole cells; (iii) synthesis of enterochelin; (iv) intracellular degradation of iron-enterochelin; and (v) synthesis of three major outer membrane proteins (OM1, OM2, and OM3). In contrast, in the wild-type strain these properties were expressed only after growth in iron-deficient medium. It is proposed that the mutation affects a gene responsible for regulating expression of the structural genes for the components of the high-affinity iron uptake systems. The term fur, for iron (Fe) uptake regulation, is suggested for this new class of mutant.