Metabolic Consequences of a Block in the Synthesis of 5-Keto- d -Fructose in a Mutant of Gluconobacter cerinus

Abstract

A mutant of Gluconobacter cerinus var. ammoniacus , IFO 3267, has been isolated which is deficient with respect to fructose 5-dehydrogenase, the enzyme catalyzing the oxidation of d -fructose to 5-keto- d -fructose (5 KF). Growth of this mutant on fructose as the sole carbon source was impaired unless the culture medium was supplemented with 5 KF. Significant randomization of the 1 and 6 positions of fructose has been reported previously for the wild-type organism during growth on this ketohexose. The pattern of 3 H incorporation into the C5 position of ribonucleic acid-ribose when the mutant was grown on [1- 3 H]fructose and [6- 3 H]fructose in the presence of 5 KF indicated that such randomization did not occur in this variant. The randomization observed in the wild type is, therefore, a consequence of the partial oxidation of fructose to the symmetrical 5 KF intermediate prior to its conversion to pentose. When the mutant was grown on [1- 3 H]fructose in the presence of unlabeled 5 KF, [5- 3 H]fructose appeared in the culture medium. Thus, 5 KF served as the oxidant for the nicotinamide adenine dinucleotide phosphate, reduced form, generated during growth on fructose.