Rapid Detection of KPC, NDM, and OXA-48-Like Carbapenemases by Real-Time PCR from Rectal Swab Surveillance Samples-Reference-Cited by-同舟云学术

Rapid Detection of KPC, NDM, and OXA-48-Like Carbapenemases by Real-Time PCR from Rectal Swab Surveillance Samples

Published:2015-05-27 Issue:8 Volume:53 Page:2731-2733
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J. Clin. Microbiol.

Author:

Lee Tracy D.,Adie Kathleen,McNabb Alan,Purych Dale,Mannan Kulvinder,Azana Robert,Ng Corrinne,Tang Patrick,Hoang Linda M. N.

Abstract

We describe a multiplex real-time PCR assay for use on the ABI 7500 Fast TaqMan platform to detect all currently described Klebsiella pneumoniae carbapenemases (KPC), New Delhi metallo-β-lactamases (NDM), and the OXA-48-like family of carbapenemases from bacterial culture lysates or sample enrichment broth lysates.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference17 articles.

1. Centers for Disease Control and Prevention. 2011. Multiplex real-time PCR detection of Klebsiella pneumoniae carbapenemase (KPC) and New Delhi metallo-β-lactamase (NDM-1). http://www.cdc.gov/HAI/settings/lab/kpc-ndm1-lab-protocol.html.

2. Rapid and simultaneous detection of genes encoding Klebsiella pneumoniae carbapenemase (blaKPC) and New Delhi metallo-β-lactamase (blaNDM) in Gram-negative bacilli;J Clin Microbiol,2013

3. Rapid detection of blaKPC carbapenemase genes by real-time PCR;J Clin Microbiol,2008

4. Detection of carbapenemases by real-time PCR and melt curve analysis on the BD Max system;J Clin Microbiol,2014

5. Real-time detection of blaKPC in clinical samples and surveillance specimens;J Clin Microbiol,2011

Cited by 30 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Minimum inhibitory concentrations of aztreonam–avibactam, ceftazidime–avibactam and meropenem in clinical isolates of Klebsiella pneumoniae harboring carbapenemase genes;The Journal of Antibiotics;2024-07-01

2. Molecular epidemiology of Carbapenemase-encoding genes and comparative evaluation of carbapenem MIC with genotypic carbapenem resistance in Klebsiella isolates from neonatal sepsis cases;Journal of Laboratory Physicians;2024-03-30

3. The molecular detection of carbapenem markers with a two-levels amplification screening protocol: epidemiological and resistome insights;Frontiers in Microbiology;2024-02-23

4. Whole genome-based genetic insights of bla _NDM producing clinical E. coli isolates in hospital settings of Pakistant;Microbiology Spectrum;2023-10-17

5. Recent Advances in Nanotechnology for the Management of Klebsiella pneumoniae–Related Infections;Biosensors;2022-12-10