Rapid and Simultaneous Detection of Genes Encoding Klebsiella pneumoniae Carbapenemase ( bla KPC ) and New Delhi Metallo-β-Lactamase ( bla NDM ) in Gram-Negative Bacilli-Reference-Cited by-同舟云学术

Rapid and Simultaneous Detection of Genes Encoding Klebsiella pneumoniae Carbapenemase ( bla KPC ) and New Delhi Metallo-β-Lactamase ( bla NDM ) in Gram-Negative Bacilli

Published:2013-04 Issue:4 Volume:51 Page:1269-1271
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Cunningham Scott A.¹,Noorie Tabassum²,Meunier Daniele²,Woodford Neil²,Patel Robin¹³

Affiliation:

1. Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, USA

2. Antimicrobial Resistance and Healthcare Associated Infections Reference Unit, Health Protection Agency (HPA), London, United Kingdom

3. Division of Infectious Diseases, Department of Internal Medicine, Mayo Clinic, Rochester, Minnesota, USA

Abstract

ABSTRACT We present a duplex, real-time PCR assay for detection of Klebsiella pneumoniae carbapenemase ( bla KPC ) and New Delhi metallo-β-lactamase ( bla NDM ) genes. Accuracy was assessed with 158 Gram-negative bacillary isolates, including 134 carbapenemase producers. The assay had 100% sensitivity and specificity compared with reference methods and a turnaround time of 90 min.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.03062-12

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