Affiliation:
1. Department of Molecular Genetics, Lilly Research Laboratories, Indianapolis, Indiana 46285.
Abstract
A segment (hft) of bacteriophage FP43 DNA cloned into plasmid pIJ702 mediated high-frequency transduction of the resulting plasmid (pRHB101) by FP43 in Streptomyces griseofuscus. The transducing particles contained linear concatemers of plasmid DNA. Lysates of FP43 prepared on S. griseofuscus containing pRHB101 also transduced many other Streptomyces species, including several that restrict plaque formation by FP43 and at least two that produce restriction endonucleases that cut pRHB101 DNA. Transduction efficiencies in different species were influenced by the addition of anti-FP43 antiserum to the transduction plates, the temperature for cell growth before transduction, the multiplicity of infection, and the host on which the transducing lysate was prepared. FP43 lysates prepared on S. griseofuscus(pRHB101) also transduced species of Streptoverticillium, Chainia, and Saccharopolyspora.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference48 articles.
1. A method to transform the ,-lactam antibiotic producer Streptomyces wadayamensis;Acebal C.;FEMS Microbiol. Lett.,1986
2. Inhibition of restriction in Streptomyces clavuligerus by heat treatment;Bailey C. R.;J. Gen. Microbiol.,1986
3. Genetic recombination in Streptomyces fradiae by protoplast fusion and cell regeneration;Baltz R. H.;J. Gen. Microbiol.,1978
4. Baltz R. H. 1982. Genetics and biochemistry of tylosin production: a model for genetic engineering in antibiotic-producing Streptomyces p. 431 444. In A. Hollaender (ed.) Genetic engineering of microorganisms for chemicals. Plenum Publishing Corp. New York.
5. Baltz R. H. 1986. Mutation in Streptomyces p. 61-94. In S. W. Queener and L. E. Day (ed.) The bacteria vol. 9. Antibioticproducing Streptomyces. Academic Press Inc. New York.
Cited by
41 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献