In Situ Detection of the Clostridium botulinum Type C 1 Toxin Gene in Wetland Sediments with a Nested PCR Assay-Reference-Cited by-同舟云学术

In Situ Detection of the Clostridium botulinum Type C 1 Toxin Gene in Wetland Sediments with a Nested PCR Assay

Published:1999-07 Issue:7 Volume:65 Page:3240-3243
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Williamson Judy L.¹,Rocke Tonie E.¹,Aiken Judd M.²

Affiliation:

1. U.S. Fish and Wildlife Service, National Wildlife Health Center, Madison, Wisconsin 53711,1 and

2. Department of Animal Health and Biomedical Sciences, University of Wisconsin—Madison, Madison, Wisconsin 537062

Abstract

ABSTRACT A nested PCR was developed for detection of the Clostridium botulinum type C 1 toxin gene in sediments collected from wetlands where avian botulism outbreaks had or had not occurred. The C 1 toxin gene was detected in 16 of 18 sites, demonstrating both the ubiquitous distribution of C. botulinum type C in wetland sediments and the sensitivity of the detection assay.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.65.7.3240-3243.1999

Reference20 articles.

1. Detection of enteroviruses in groundwater with the polymerase chain reaction

2. Ausubel F. M. Brent R. Kingston R. E. Moore D. D. Seidman J. G. Smith J. A. Struhl K. Current protocols in molecular biology 1 1997 Greene Publishing Associates and Wiley-Interscience New York N.Y

3. PCR for detection of Clostridium botulinum type C in avian and environmental samples

4. Nucleotide sequence of Clostridium botulinum C1 neurotoxin.;Hauser D.;Nucleic Acids Res.,1990

5. Hendrickson D. A. Reagents and stains Manual of clinical microbiology 4th ed. Lennette E. H. Balows A. Hausler W. J. Jr. Shadomy H. J. 1985 1093 1107 American Society for Microbiology Washington D.C

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