Affiliation:
1. Laboratorio Alimenti, Istituto Superiore di Sanità, Rome, Italy.
Abstract
A PCR was developed and applied for the detection of Clostridium botulinum type C in 18 avian and environmental samples collected during an outbreak of avian botulism, and the results were compared with those obtained by conventional methodologies based on the mouse bioassay. PCR and mouse bioassay results compared well (100%) after the enrichment of samples, but PCR results directly indicated the presence of this microorganism in six samples, while only one of these contained the type C botulinal neurotoxin before enrichment. The PCR assay was sensitive (limit of detection between 15 and 15 x 10(3) spores per PCR), specific (no amplification products were obtained with other clostridia), and rapid, since sonicated and heated samples provided enough template for amplification without any DNA purification. Eleven isolates of C. botulinum type C were recovered from mallards (Anas platyrhynchos), grey herons (Ardea cinerea), and mud during investigation of this outbreak.
Publisher
American Society for Microbiology
Reference30 articles.
1. Two cases of type E infant botulism caused by neurotoxigenic Clostridium butyricum in Italy;Aureli P.;J. Infect. Dis.,1986
2. Benassi M. C. and E. Catelli. 1990. Episodi di botulismo in un allevamento di fagiani dell'Emilia Romagna: rilievi epidemiologici e interventi profilattici p. 239-242. In Atti XI Convegno ``Allevamenti di selvaggina '' Bastia Umbra (PG).
3. Botulism in intensively farmed chukar partridges (Alectoris chukar);Catelli E.;Zootec. Int.,1994
4. Su un focolaio di botulismo in uccelli acquatici;Corradini L.;Sel. Vet.,1977
5. Il botulismo in provincia di;Corradini L.;Ferrara. Prog. Vet.,1981
Cited by
47 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献