Application of an enzyme-linked immunosorbent assay for screening of T-2 toxin-producing Fusarium spp

Author:

Nagayama S1,Kawamura O1,Ohtani K1,Ryu J C1,Latus D1,Sudheim L1,Ueno Y1

Affiliation:

1. Department of Toxicology and Microbial Chemistry, Science University of Tokyo, Japan.

Abstract

Culture filtrates of Fusarium species were subjected without clean-up procedures to an indirect competitive enzyme-linked immunosorbent assay with anti-T-2 toxin monoclonal antibody. Fusarium sporotrichioides, F. poae, F. tricinctum, and F. culmorum strains were positive for T-2 toxin, with a minimum detection limit of 5 pg per assay (100 pg/ml of culture filtrate), and the assay data correlated well with the gas-liquid chromatographic data.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference10 articles.

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2. Preparation and characterization of monoclonal antibodies to the trichothecene mycotoxin T-2;Hunter K. W.;Appl. Environ. Microbiol.,1985

3. Detection of ochratoxin A and T-2 toxin by one step ELISA;Itoh Y.;Proc. Jpn. Assoc. Mycotoxicol.,1986

4. Enzyme-linked immunosorbent assay of ochratoxin A in barley;Morgan M. R. A.;J. Assoc. Off. Anal. Chem.,1983

5. Metabolism of trichothecene mycotoxins. I. Microsomal deacetylation of T-2 toxin in animal tissues;Ohta M.;J. Biochem. (Tokyo),1977

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