C. Diff Quik Chek Complete Enzyme Immunoassay Provides a Reliable First-Line Method for Detection of Clostridium difficile in Stool Specimens-Reference-Cited by-同舟云学术

C. Diff Quik Chek Complete Enzyme Immunoassay Provides a Reliable First-Line Method for Detection of Clostridium difficile in Stool Specimens

Published:2010-02 Issue:2 Volume:48 Page:603-605
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Quinn Criziel D.¹,Sefers Susan E.¹,Babiker Wisal²,He Ying¹,Alcabasa Romina²,Stratton Charles W.¹³,Carroll Karen C.²,Tang Yi-Wei¹³

Affiliation:

1. Departments of Pathology

2. Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287

3. Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232

Abstract

ABSTRACT We evaluated a single membrane device assay for simultaneously detecting both Clostridium difficile glutamate dehydrogenase (GDH) and toxin A/B antigens against a standard that combines two PCR assays and cytotoxigenic culture. Results showing dual GDH and toxin A/B antigen positives and negatives can be reported immediately as true positives and negatives, respectively. Specimens with discrepant results for GDH and toxins A/B, which comprised 13.2% of the specimens, need to be retested.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01614-09

Reference18 articles.

1. Chow, W. H., C. McCloskey, Y. Tong, L. Hu, Q. You, C. P. Kelly, H. Kong, Y. W. Tang, and W. Tang. 2008. Application of isothermal helicase-dependent amplification with a disposable detection device in a simple sensitive stool test for toxigenic Clostridium difficile. J. Mol. Diagn. 10 : 452-458.

2. Is a Two-Step Glutamate Dehyrogenase Antigen-Cytotoxicity Neutralization Assay Algorithm Superior to the Premier Toxin A and B Enzyme Immunoassay for Laboratory Detection of Clostridium difficile ?

3. Measurement of Human Cytomegalovirus Loads by Quantitative Real-Time PCR for Monitoring Clinical Intervention in Transplant Recipients

4. Multicenter Evaluation of the Clostridium difficile TOX A/B TEST

5. Detection of Clostridium difficile Toxin: Comparison of Enzyme Immunoassay Results with Results Obtained by Cytotoxicity Assay

Cited by 99 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Molecular methods for healthcare-acquired infections;Diagnostic Molecular Pathology;2024

2. Evaluation of Two Rapid Diagnostic Clostridioides difficile Infection Tests in a Chinese Hospital: A Real-world Analysis;Jundishapur Journal of Microbiology;2022-11-26

3. Isolation of Clostridioides difficile PCR Ribotype 027 from single-use hospital gown ties;Journal of Medical Microbiology;2022-06-08

4. Laboratory diagnosis of Clostridioides (Clostridium) difficile infection in domestic animals: A short review;Anaerobe;2022-06

5. Rapid Quantification of C. difficile Glutamate Dehydrogenase and Toxin B (TcdB) with a NanoBiT Split-Luciferase Assay;Analytical Chemistry;2022-05-28