Hemin levels in culture medium of Porphyromonas (Bacteroides) gingivalis regulate both hemin binding and trypsinlike protease production

Author:

Carman R J1,Ramakrishnan M D1,Harper F H1

Affiliation:

1. Medical Research Council Dental Research Unit, London Hospital Medical College, England.

Abstract

Washed cells and Sarkosyl-insoluble outer membrane preparations of the black-pigmented bacteroides Porphyromonas gingivalis W50 bound hemin. The amount of hemin removed from a buffered solution by both cells and outer membranes was significantly larger if bacteria had been grown in broths supplemented with 5 mg of hemin per liter rather than none. Conversely, cells grown without supplemental hemin bound relatively little. However, all preparations bound some hemin. In addition, hemin regulated the production of significantly higher levels of trypsinlike protease by P. gingivalis W50. The nonpigmented variant, W50 BE1, showed no such responses to the levels of hemin in the growth medium.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Reference28 articles.

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3. .Carman R. J. M. D. Ramakrishnan F. H. Harper and M. A. Curtis. 1990. Bacteroides gingivalis: role of haemin and iron in aetiology of destructive periodontitis p. 161-167. In S. P. Borriello (ed.) Clinical and molecular aspects of anaerobes. Wrightson Biomedical Publishing Petersfield England.

4. Congo red binding phenotype is associated with hemin binding and increased infectivity of Shigella flexneri in the HeLa cell model;Daskaleros P. A.;Infect. Immun.,1987

5. Haptoglobin: a natural bacteriostat;Eaton J. W.;Science,1982

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