Core Genome Multilocus Sequence Typing and Prediction of Antimicrobial Susceptibility Using Whole-Genome Sequences of Escherichia coli Bloodstream Infection Isolates-Reference-Cited by-同舟云学术

Core Genome Multilocus Sequence Typing and Prediction of Antimicrobial Susceptibility Using Whole-Genome Sequences of Escherichia coli Bloodstream Infection Isolates

Published:2021-10-18 Issue:11 Volume:65 Page:
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Banerjee Ritu¹,Cunningham Scott A.²,Beisken Stephan³,Posch Andreas E.³,Johnston Brian⁴,Johnson James R.⁴,Patel Robin²^ORCID

Affiliation:

1. Vanderbilt University, Nashville, Tennessee, USA

2. Mayo Clinic, Rochester, Minnesota, USA

3. Ares Genetics GmbH, Vienna, Austria

4. Veterans Affairs Medical Center, University of Minnesota, Minneapolis, Minnesota, USA

Abstract

In total, 50 Escherichia coli bloodstream isolates from the clinical laboratory and 12 E. coli isolates referred for pulsed-field gel electrophoresis (PFGE) were sequenced, assessed for clonality using core genome multilocus sequence typing (cgMLST), and evaluated for genomic susceptibility predictions using ARESdb. Results of sequence typing using whole-genome sequencing (WGS)-based MLST and sequence type (ST)-specific PCR were identical.

Funder

HHS | NIH | National Institute of Allergy and Infectious Diseases

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.01139-21

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