Design and Evaluation of Useful Bacterium-Specific PCR Primers That Amplify Genes Coding for Bacterial 16S rRNA-Reference-Cited by-同舟云学术

Design and Evaluation of Useful Bacterium-Specific PCR Primers That Amplify Genes Coding for Bacterial 16S rRNA

Published:1998-02 Issue:2 Volume:64 Page:795-799
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Marchesi Julian R.¹,Sato Takuichi²,Weightman Andrew J.¹,Martin Tracey A.¹,Fry John C.¹,Hiom Sarah J.³,Wade William G.⁴

Affiliation:

1. School of Pure and Applied Biology, University of Wales College of Cardiff, Cardiff CF1 3TL,1

2. Department of Oral Microbiology, Niigata University School of Dentistry, Niigata 951, Japan2

3. Department of Oral Medicine, Pathology and Microbiology, Centre for the Study of Oral Disease, Bristol Dental Hospital and School, Bristol BS1 2LY,3 and

4. Department of Oral Medicine and Pathology, Guys and St. Thomas’s Medical and Dental School, Guy’s Hospital, London SE1 9RT,4United Kingdom, and

Abstract

ABSTRACT We report the design and evaluation of PCR primers 63f and 1387r for amplification of 16S rRNA genes from bacteria. Their specificity and efficacy were tested systematically with a variety of bacterial species and environmental samples. They were found to be more useful for 16S rRNA gene amplification in ecological and systematic studies than PCR amplimers that are currently more generally used.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.64.2.795-799.1998

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