Application of Real-Time Fluorescent PCR for Quantitative Assessment of Neospora caninum Infections in Organotypic Slice Cultures of Rat Central Nervous System Tissue-Reference-Cited by-同舟云学术

Application of Real-Time Fluorescent PCR for Quantitative Assessment of Neospora caninum Infections in Organotypic Slice Cultures of Rat Central Nervous System Tissue

Published:2002-01 Issue:1 Volume:40 Page:252-255
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Müller Norbert¹,Vonlaufen Nathalie¹,Gianinazzi Christian²,Leib Stephen L.²,Hemphill Andrew¹

Affiliation:

1. Institute of Parasitology, University of Berne, Länggass-Strasse 122, 3012 Berne

2. Institute for Infectious Diseases, University of Berne, Friedbühlstrasse 51, 3010 Berne, Switzerland

Abstract

ABSTRACT The previously described Nc5-specific PCR test for the diagnosis of Neospora caninum infections was used to develop a quantitative PCR assay which allows the determination of infection intensities within different experimental and diagnostic sample groups. The quantitative PCR was performed by using a dual fluorescent hybridization probe system and the LightCycler Instrument for online detection of amplified DNA. This assay was successfully applied for demonstrating the parasite proliferation kinetics in organotypic slice cultures of rat brain which were infected in vitro with N. caninum tachyzoites. This PCR-based method of parasite quantitation with organotypic brain tissue samples can be regarded as a novel ex vivo approach for exploring different aspects of cerebral N. caninum infection.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.40.1.252-255.2002

Reference13 articles.

1. Dubey, J. P. 2000. Neosporosis—the first decade of research. Int. J. Parasitol.29:1485–1488.

2. Dubey, J. P., and A. De Lahunta. 1993. Neosporosis associated congenital limb deformities in a calf. Appl. Parasitol.34:229–233.

3. Dubey, J. P, and D. S. Lindsay. 1996. A review of Neospora caninum and neosporosis. Vet. Parasitol.67:1–59.

4. Ellis, J. T. 1998. Polymerase chain reaction approaches for the detection of Neospora caninum and Toxoplasma gondii. Int. J. Parasitol.28:1053–1060.

5. Gottstein, B., B. Hentrich, R. Wyss, B. Thür, A. Busato, K. D. C. Stärk, and N. Müller. 1998. Molecular and immunodiagnostic investigations on bovine neosporosis in Switzerland. Int. J. Parasitol.28:679–691.

Cited by 57 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Efficacy of the bumped kinase inhibitor BKI-1708 against the cyst-forming apicomplexan parasites Toxoplasma gondii and Neospora caninum in vitro and in experimentally infected mice;International Journal for Parasitology: Drugs and Drug Resistance;2024-08

2. Working towards the development of vaccines and chemotherapeutics against neosporosis—With all of its ups and downs—Looking ahead;Advances in Parasitology;2024

3. In vitro screening technologies for the discovery and development of novel drugs against Toxoplasma gondii;Expert Opinion on Drug Discovery;2023-11-03

4. In Vitro versus in Mice: Efficacy and Safety of Decoquinate and Quinoline-O-Carbamate Derivatives against Experimental Infection with Neospora caninum Tachyzoites;Pathogens;2023-03-13

5. Immunization with a Multivalent Listeria monocytogenes Vaccine Leads to a Strong Reduction in Vertical Transmission and Cerebral Parasite Burden in Pregnant and Non-Pregnant Mice Infected with Neospora caninum;Vaccines;2023-01-11