Impact of Nasopharyngeal Swab Types on Detection of Bordetella pertussis by PCR and Culture-Reference-Cited by-同舟云学术

Impact of Nasopharyngeal Swab Types on Detection of Bordetella pertussis by PCR and Culture

Published:2002-10 Issue:10 Volume:40 Page:3838-3840
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Cloud Joann L.¹,Hymas Weston²,Carroll Karen C.¹³

Affiliation:

1. Associated Regional and University Pathologists (ARUP) Institute for Clinical and Experimental Pathology

2. ARUP Molecular Infectious Diseases Laboratory

3. Department of Pathology, University of Utah, Salt Lake City, Utah

Abstract

ABSTRACT A study was designed to assess the performance of various swabs and transport media routinely used to collect specimens submitted for Bordetella culture and PCR. Calcium-alginate swabs inhibited the PCR. No inhibition was detected in any PCRs with dacron or rayon swabs. All swab materials performed similarly for recovery of Bordetella pertussis in culture. The Amies with charcoal transport system performed poorly for culture. Calcium-alginate swabs are not recommended for PCR-based detection of B. pertussis . Dacron and rayon swabs are an excellent choice for both PCR and culture.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.40.10.3838-3840.2002

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