Method for Inactivating and Fixing Unstained Smear Preparations of Mycobacterium tuberculosis for Improved Laboratory Safety-Reference-Cited by-同舟云学术

Method for Inactivating and Fixing Unstained Smear Preparations of Mycobacterium tuberculosis for Improved Laboratory Safety

Published:2002-11 Issue:11 Volume:40 Page:4077-4080
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Chedore Pamela¹,Th'ng Cecelia¹,Nolan Dennis H.²,Churchwell George M.²,Sieffert David E.²,Hale Yvonne M.²,Jamieson Frances¹

Affiliation:

1. Laboratories Branch, Ontario Ministry of Health and Long-Term Care, Toronto, Ontario, Canada

2. Florida Department of Health, Bureau of Laboratories, Jacksonville, Florida

Abstract

ABSTRACT The inactivation of smears that contain Mycobacterium tuberculosis for microscopy before removal of the material from a biosafety cabinet is an important safety factor in preventing the potential transmission of tuberculosis to laboratory workers. The fixing and inactivating properties of heat flaming, 70% ethanol, and 1, 3, and 5% phenol in ethanol for smears containing M. tuberculosis were investigated. Heat flaming failed to inactivate the smear material, whereas 5% phenol in ethanol successfully fixed and inactivated all smears containing M. tuberculosis both from concentrated sputum samples and from culture material.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.40.11.4077-4080.2002

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