Biosafety and Proteome Profiles of Different Heat Inactivation Methods for Mycobacterium tuberculosis-Reference-Cited by-同舟云学术

Biosafety and Proteome Profiles of Different Heat Inactivation Methods for Mycobacterium tuberculosis

Published:2021-12-22 Issue:3 Volume:9 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Wang Cheng-Hui¹²³,Putri Denise Utami³⁴,Lee Jau-Ching¹,Liao Chi-Chih¹,Tsao Sung-tzu¹,Hsiao Ai-Lin¹,Wu Jhao-Hui¹,Chen Xiao-Wei⁴,Lee Chih-Hsin³⁴⁵,Tsai I-Lin³⁶⁷⁸⁹^ORCID

Affiliation:

1. Department of Laboratory Medicine, Wan Fang Hospital, Taipei Medical University, Taipei, Taiwan

2. School of Medical Laboratory Science and Biotechnology, College of Medical Science and Technology, Taipei Medical University, Taipei, Taiwan

3. Pulmonary Research Center, Wan Fang Hospital, Taipei Medical University, Taipei, Taiwan

4. Division of Pulmonary Medicine, Department of Internal Medicine, Wan Fang Hospital, Taipei Medical University, Taipei, Taiwan

5. Division of Pulmonary Medicine, Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan

6. Department of Biochemistry and Molecular Cell Biology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan

7. Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan

8. Master Program in Clinical Genomics and Proteomics, School of Pharmacy, Taipei Medical University, Taipei, Taiwan

9. International PhD Program for Cell Therapy and Regeneration Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan

Abstract

Inactivation of Mycobacterium tuberculosis is an important step to guarantee biosafety for subsequent M. tuberculosis identification and related research, notably in areas of endemicity with minimal resources. However, certain biomolecules might be denatured or hydrolyzed because of the harsh inactivation process, and a standardized protocol is yet to be determined.

Funder

Wan Fang Hospital, Taipei Medical University

Higher Education Sprout Project of Ministry of Education in Taiwan

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.00716-21

Reference43 articles.

1. Building a tuberculosis-free world: The Lancet Commission on tuberculosis

2. Molecular Biology of Drug Resistance in Mycobacterium tuberculosis

3. Proteomics for the Investigation of Mycobacteria

4. Systems proteomics approaches to study bacterial pathogens: application to Mycobacterium tuberculosis

5. A novel protein extraction method for identification of mycobacteria using MALDI-ToF MS

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