Collaborative Evaluation of Optimal Antifungal Susceptibility Testing Conditions for Dermatophytes-Reference-Cited by-同舟云学术

Collaborative Evaluation of Optimal Antifungal Susceptibility Testing Conditions for Dermatophytes

Published:2002-11 Issue:11 Volume:40 Page:3999-4003
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Fernández-Torres Belkys¹,Cabañes Francisco J.²,Carrillo-Muñoz Alfonso J.³,Esteban Alexandre²,Inza Isabel¹,Abarca Lourdes²,Guarro Josep¹

Affiliation:

1. Unitat de Microbiología, Facultat de Medicina, Universitat Rovira i Virgili, Reus

2. Departament de Sanitat i d'Anatomia Animals, Facultat de Veterinaria, Universitat Autònoma de Barcelona, Bellaterra

3. Departament of Microbiología, Asesoría Científica y de Investigación Aplicada, Barcelona, Spain

Abstract

ABSTRACT A multicenter study was conducted to define the most suitable testing conditions for antifungal susceptibility of dermatophytes. Broth microdilution MICs of clotrimazole, itraconazole, and terbinafine were determined in three centers against 60 strains of dermatophytes. The effects of inoculum density (ca. 10 3 and 10 4 CFU/ml), incubation time (3, 7, and 14 days), endpoint criteria for MIC determination (complete [MIC-0] and prominent [MIC-2] growth inhibition), and incubation temperature (28 and 37°C) on intra- and interlaboratory agreement were analyzed. The optimal testing conditions identified were an inoculum of 10 4 CFU/ml, a temperature of incubation of 28°C, an incubation period of 7 days, and MIC-0.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.40.11.3999-4003.2002

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