Affiliation:
1. Microbiology Laboratory
2. Department of Pediatrics, Hôpital Necker-Enfants Malades, Paris
3. Microbiology Laboratory, Hôpital Raymond Poincaré, Garches, France
Abstract
ABSTRACT
During 1999, we used partial 16S rRNA gene sequencing for the prospective identification of atypical nonfermenting gram-negative bacilli isolated from patients attending our cystic fibrosis center. Of 1,093 isolates of nonfermenting gram-negative bacilli recovered from 148 patients, 46 (4.2%) gave problematic results with conventional phenotypic tests. These 46 isolates were genotypically identified as
Pseudomonas aeruginosa
(19 isolates, 12 patients),
Achromobacter xylosoxidans
(10 isolates, 8 patients),
Stenotrophomonas maltophilia
(9 isolates, 9 patients),
Burkholderia cepacia
genomovar I/III (3 isolates, 3 patients),
Burkholderia vietnamiensis
(1 isolate),
Burkholderia gladioli
(1 isolate), and
Ralstonia mannitolilytica
(3 isolates, 2 patients), a recently recognized species.
Publisher
American Society for Microbiology
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49 articles.
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